Coding

Part:BBa_K3788013:Design

Designed by: Rebecca Pagès   Group: iGEM21_Aix-Marseille   (2021-09-27)


IPTG inducible promoter with RBS, PelB_sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 280
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 305


Design Notes

After the sequence eventual mutation was prevented : Amino acids have been changed to avoid the formation of a stop codon and also to remove restriction sites corresponding to RFC10 and RFC25.


Source

This composite part was created thanks to BBa_J04500 in fusion to BBa_K3788012

References